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PXD028613-1

PXD028613 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMuc2-dependent microbial colonization of the jejunal mucus layer is diet sensitive and confers resistance to enteric pathogen infection
DescriptionObesity is associated with an increased risk of mucosal infections; however, the mechanistic basis of this phenomenon remains incompletely defined. Intestinal mucus barrier systems normally prevent infections, but are sensitive to changes in the luminal environment. Here we demonstrate that mice exposed to an obesogenic Western-style diet (WSD) suffer regiospecific failure of the mucus barrier in the small intestinal jejunum caused by diet-induced mucus condensation, which occurs independently of microbiota alterations. Mucus barrier disruption due to either WSD exposure or chromosomal Muc2 deletion results in collapse of the commensal jejunal microbiota, which in turn sensitises mice to atypical jejunal colonization by the enteric pathogen Citrobacter rodentium. We identify the jejunal mucus layer as a microbial habitat, and link the regiospecific mucus dependency of the microbiota to fundamental properties of the jejunal niche. Together, our data identifies a symbiotic mucus-microbiota relationship that normally prevents jejunal pathogen colonization, but is highly sensitive to disruption by exposure to a Western-style diet.
HostingRepositoryPRIDE
AnnounceDate2023-05-12
AnnouncementXMLSubmission_2023-05-12_07:20:43.090.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLiisaArike
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-09-17 09:10:01ID requested
12023-05-12 07:20:43announced
22023-11-14 08:53:28announced2023-11-14: Updated project metadata.
Publication List
Keyword List
submitter keyword: proteomics, Q-Exactive, obesity,Mucus, mouse
Contact List
LiisaArike
contact affiliationProteomics laboratory, Mucin Biology Groups, Department of Medical Biochemistry and Cell Biology, University of Gothenburg, Sweden
contact emailliisaarike@gmail.com
lab head
LiisaArike
contact affiliationPhD
contact emailliisaarike@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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