<<< Full experiment listing

PXD028590-2

PXD028590 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAmphiregulin mediates non-cell-autonomous effect of senescence on reprogramming
DescriptionCellular senescence is an irreversible growth arrest with a highly dynamic secretome, termed the senescence-associated secretory phenotype (SASP). Senescence has been implicated in somatic reprogramming to pluripotency. The cell-intrinsic proliferation arrest is a barrier for reprogramming, whereas the SASP facilitates the cell fate conversion in nonsenescent cells. However, the mechanisms by which reprogramming-induced senescence regulates cell plasticity are not well understood. Here, we have further investigated how the heterogeneity of paracrine senescence impacts reprogramming. We show that senescence promotes in vitro reprogramming in a stress-dependent manner. We identified a catalog of SASP factors and pathways potentially involved in the cell fate conversion using an unbiased proteomic analysis. Amphiregulin (AREG), a growth factor frequently secreted by the senescent cells, promotes in vitro reprogramming by accelerating proliferation and MET via the EGFR signaling pathway. Of note, AREG treatment diminished the negative effect of donor age on reprogramming. Finally, AREG enhances in vivo reprogramming in the skeletal muscle. Hence, senescence could facilitate cellular plasticity via various SASP factors to promote reprogramming and tissue repair.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:53:41.218.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterThibaut Douché
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListL-methionine (S)-sulfoxide; mono N-acetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-09-16 09:00:53ID requested
12022-07-28 04:44:35announced
22023-11-14 08:53:41announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: shotgun proteomics,Mouse, cell culture, secretome, senescence
Contact List
Han Li
contact affiliationPlasticité cellulaire et modélisation des maladies, CNRS UMR 3738, Institut Pasteur, Paris, France
contact emailhan.li@pasteur.fr
lab head
Thibaut Douché
contact affiliationInstitut Pasteur
contact emailthibaut.douche@pasteur.fr
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/07/PXD028590
PRIDE project URI
Repository Record List
[ + ]