PXD028435 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Single muscle fiber proteomics reveals remodeling of focal adhesions in response to muscle unloading and microgravity |
Description | Spaceflight causes loss of muscle mass and strength, metabolic remodeling and insulin resistance, contributing to severe challenges for astronaut health. We used highly sensitive proteomics to detail single fiber type-specific molecular remodeling caused by muscle unloading in subjects undergoing prolonged bed rest. We then measured the muscle proteome of astronauts before and after a mission on the ISS. Our combined datasets show downregulation of complexes involved in fiber-matrix interaction, force transmission and insulin receptor stabilization on the sarcolemma. Unloading upregulated anti-oxidant responses in slow but not in fast fibers, markers of neuromuscular damage and the hypusination pathway that uniquely modifies translation initiation factor 5A. These muscle intrinsic changes in adhesion, protein turnover and redox balance may contribute to the whole-body detrimental effects caused by prolonged unloading. We conclude that single muscle fiber analysis by proteomics can support the development of advanced countermeasures targeting the mechano-metabolic molecular axis. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:39:56.077.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Mario Oroshi |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | timsTOF Pro |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-09-09 13:53:42 | ID requested | |
1 | 2023-03-11 01:32:46 | announced | |
⏵ 2 | 2023-11-14 08:39:58 | announced | 2023-11-14: Updated project metadata. |
Publication List
Murgia M, Ciciliot S, Nagaraj N, Reggiani C, Schiaffino S, Franchi MV, Pi, š, ot R, Š, imuni, č B, Toniolo L, Blaauw B, Sandri M, Biolo G, Fl, ü, ck M, Narici MV, Mann M, Signatures of muscle disuse in spaceflight and bed rest revealed by single muscle fiber proteomics. PNAS Nexus, 1(3):pgac086(2022) [pubmed] |
Keyword List
submitter keyword: proteomics, microgravity, muscle |
Contact List
Marta Murgia |
contact affiliation | Max Planck Institute of Biochemistry Department of Proteomics and Signal Transduction Munich, Germany |
contact email | mmurgia@biochem.mpg.de |
lab head | |
Mario Oroshi |
contact affiliation | Proteomics |
contact email | oroshi@biochem.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD028435
- Label: PRIDE project
- Name: Single muscle fiber proteomics reveals remodeling of focal adhesions in response to muscle unloading and microgravity