PXD028435

PXD028435 is an original dataset announced via ProteomeXchange.

Title	Single muscle fiber proteomics reveals remodeling of focal adhesions in response to muscle unloading and microgravity
Description	Spaceflight causes loss of muscle mass and strength, metabolic remodeling and insulin resistance, contributing to severe challenges for astronaut health. We used highly sensitive proteomics to detail single fiber type-specific molecular remodeling caused by muscle unloading in subjects undergoing prolonged bed rest. We then measured the muscle proteome of astronauts before and after a mission on the ISS. Our combined datasets show downregulation of complexes involved in fiber-matrix interaction, force transmission and insulin receptor stabilization on the sarcolemma. Unloading upregulated anti-oxidant responses in slow but not in fast fibers, markers of neuromuscular damage and the hypusination pathway that uniquely modifies translation initiation factor 5A. These muscle intrinsic changes in adhesion, protein turnover and redox balance may contribute to the whole-body detrimental effects caused by prolonged unloading. We conclude that single muscle fiber analysis by proteomics can support the development of advanced countermeasures targeting the mechano-metabolic molecular axis.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:39:56.077.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mario Oroshi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-09 13:53:42	ID requested
1	2023-03-11 01:32:46	announced
⏵ 2	2023-11-14 08:39:58	announced	2023-11-14: Updated project metadata.

Murgia M, Ciciliot S, Nagaraj N, Reggiani C, Schiaffino S, Franchi MV, Pi, š, ot R, Š, imuni, č B, Toniolo L, Blaauw B, Sandri M, Biolo G, Fl, ü, ck M, Narici MV, Mann M, Signatures of muscle disuse in spaceflight and bed rest revealed by single muscle fiber proteomics. PNAS Nexus, 1(3):pgac086(2022) [pubmed]

submitter keyword: proteomics, microgravity, muscle

Marta Murgia
contact affiliation	Max Planck Institute of Biochemistry Department of Proteomics and Signal Transduction Munich, Germany
contact email	mmurgia@biochem.mpg.de
lab head
Mario Oroshi
contact affiliation	Proteomics
contact email	oroshi@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD028435

PRIDE project URI

• PRIDE
  1. PXD028435
     1. Label: PRIDE project
     2. Name: Single muscle fiber proteomics reveals remodeling of focal adhesions in response to muscle unloading and microgravity