PXD028391

PXD028391 is an original dataset announced via ProteomeXchange.

Title	CsrA-controlled proteins important for Acinetobacter baumannii desiccation tolerance
Description	Hospital environments serve as excellent reservoirs for the opportunistic pathogen Acinetobacter baumannii in part because it is exceptionally tolerant to desiccation. To understand the functional basis this trait, we used transposon sequencing (Tn-seq) to identify genes contributing to desiccation tolerance in A. baumannii strain AB5075. We identified 142 candidate desiccation tolerance genes, one of which encoded the global post-transcriptional regulator CsrA. We characterized CsrA in more detail by using proteomics to identify proteins that were differentially present in wild type and csrA mutant cells. Among these were a predicted universal stress protein A, an iron-containing redox protein, a KGG-domain containing protein, and catalase. Subsequent mutant analysis showed that each of these proteins was required for A. baumannii desiccation tolerance. The amino acid sequence of the KGG-domain containing protein predicts that it is an intrinsically disordered protein. Such proteins are critical for desiccation tolerance of the small animals called tardigrades. This protein also has a repeat nucleic acid binding amino acid motif, suggesting that it may protect A. baumannii DNA from desiccation-induced damage.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:26:56.602.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaoting Tang
SpeciesList	scientific name: Acinetobacter baumannii AB5075; NCBI TaxID: 1116234;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-09 08:34:57	ID requested
1	2022-10-13 10:37:46	announced
⏵ 2	2023-11-14 08:26:58	announced	2023-11-14: Updated project metadata.

Oda Y, Shapiro MM, Lewis NM, Zhong X, Huse HK, Zhong W, Bruce JE, Manoil C, Harwood CS, CsrA-Controlled Proteins Reveal New Dimensions of Acinetobacter baumannii Desiccation Tolerance. J Bacteriol, 204(4):e0047921(2022) [pubmed]

submitter keyword: Acinetobacter baumannii, intrinsically disordered proteins, CsrA, desiccation, Tn-seq

Caroline S. Harwood
contact affiliation	1Department of Microbiology, University of Washington, Seattle, Washington, USA
contact email	csh5@uw.edu
lab head
Xiaoting Tang
contact affiliation	University of Washington
contact email	xiaoting@uw.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD028391

PRIDE project URI

• PRIDE
  1. PXD028391
     1. Label: PRIDE project
     2. Name: CsrA-controlled proteins important for Acinetobacter baumannii desiccation tolerance