PXD028314 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | PCNA regulates metabolism by stabilizing ENO1 and 6PGD |
Description | The essential roles of PCNA as a scaffold protein in DNA replication and repair are well established, while its more recently discovered cytosolic roles are less explored. Alpha-enolase (ENO1) and 6-phosphogluconate dehydrogenase (6PGD), important proteins in glycolysis and the Pentose Phosphate Pathway (PPP), respectively, both contain PCNA interacting motifs. Here we show reduced binding to PCNA when the PCNA interacting motif APIM in ENO1 is mutated. Mutant cells have lower ENO1 protein levels, have reduced glucose consumption, altered glycolytic metabolite and nucleoside phosphate pools and increased activation of the citric acid cycle (TCA) compared to parental cells. Treatment of a panel of haematological cell lines with a cell penetrating peptide containing APIM (ATX-101), lead to a metabolic shift with reduction in glycolytic metabolite and nucleoside phosphate pools as well reduced levels of ENO1 and 6PGD proteins. These results suggests that PCNA stabilize ENO1 and 6PGD, and that targeting PCNA reduce the glycolysis, PPP and nucleoside phosphate pools via destabilization of these proteins. |
HostingRepository | PRIDE |
AnnounceDate | 2023-03-10 |
AnnouncementXML | Submission_2023-03-10_14:46:08.275.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | AnimeshSharma |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-09-09 08:21:33 | ID requested | |
⏵ 1 | 2023-03-10 14:46:09 | announced | |
2 | 2023-11-14 08:33:38 | announced | 2023-11-14: Updated project metadata. |
Publication List
R, ø, st LM, R, æ, der SB, Olaisen C, S, ø, gaard CK, Sharma A, Bruheim P, Otterlei M, PCNA regulates primary metabolism by scaffolding metabolic enzymes. Oncogene, 42(8):613-624(2023) [pubmed] |
Keyword List
submitter keyword: glycolysis, PI3K/AKT pathway, cell signaling, APIM-peptide, ATX-101,APIM |
Contact List
MaritOtterlei |
contact affiliation | Department of Clinical and Molecular Medicine, Norwegian University of Science and Technology (NTNU), PO Box 8905, N-7491, Trondheim, Norway Phone: +47 72573075, Fax: +47 72576400 |
contact email | marit.otterlei@ntnu.no |
lab head | |
AnimeshSharma |
contact affiliation | Engineer at NTNU, Norway |
contact email | sharma.animesh@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD028314
- Label: PRIDE project
- Name: PCNA regulates metabolism by stabilizing ENO1 and 6PGD