PXD028314

PXD028314 is an original dataset announced via ProteomeXchange.

Title	PCNA regulates metabolism by stabilizing ENO1 and 6PGD
Description	The essential roles of PCNA as a scaffold protein in DNA replication and repair are well established, while its more recently discovered cytosolic roles are less explored. Alpha-enolase (ENO1) and 6-phosphogluconate dehydrogenase (6PGD), important proteins in glycolysis and the Pentose Phosphate Pathway (PPP), respectively, both contain PCNA interacting motifs. Here we show reduced binding to PCNA when the PCNA interacting motif APIM in ENO1 is mutated. Mutant cells have lower ENO1 protein levels, have reduced glucose consumption, altered glycolytic metabolite and nucleoside phosphate pools and increased activation of the citric acid cycle (TCA) compared to parental cells. Treatment of a panel of haematological cell lines with a cell penetrating peptide containing APIM (ATX-101), lead to a metabolic shift with reduction in glycolytic metabolite and nucleoside phosphate pools as well reduced levels of ENO1 and 6PGD proteins. These results suggests that PCNA stabilize ENO1 and 6PGD, and that targeting PCNA reduce the glycolysis, PPP and nucleoside phosphate pools via destabilization of these proteins.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:33:37.605.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Animesh Sharma
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-09 08:21:33	ID requested
1	2023-03-10 14:46:09	announced
⏵ 2	2023-11-14 08:33:38	announced	2023-11-14: Updated project metadata.

R, ø, st LM, R, æ, der SB, Olaisen C, S, ø, gaard CK, Sharma A, Bruheim P, Otterlei M, PCNA regulates primary metabolism by scaffolding metabolic enzymes. Oncogene, 42(8):613-624(2023) [pubmed]

submitter keyword: glycolysis, PI3K/AKT pathway, cell signaling, APIM-peptide, ATX-101,APIM

Marit Otterlei
contact affiliation	Department of Clinical and Molecular Medicine, Norwegian University of Science and Technology (NTNU), PO Box 8905, N-7491, Trondheim, Norway Phone: +47 72573075, Fax: +47 72576400
contact email	marit.otterlei@ntnu.no
lab head
Animesh Sharma
contact affiliation	Engineer at NTNU, Norway
contact email	sharma.animesh@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD028314

PRIDE project URI

• PRIDE
  1. PXD028314
     1. Label: PRIDE project
     2. Name: PCNA regulates metabolism by stabilizing ENO1 and 6PGD