PXD028092 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Transcriptome-wide identification of coding and noncoding RNA-binding proteins defines the extended RNA interactome of Leishmania mexicana |
Description | Non-poly(A) RNA molecules including noncoding RNAs (ncRNAs) comprise the major portion of the total transcribed molecules in the cell. In addition to the mRNAs the ncRNAs also function as ribonucleoprotein particles (RNPs) and carry out biological functions including synthesis of new proteins, RNA processing, genome remodelling and regulation of transcription. We therefore envisaged a comprehensive transcriptome-wide identification of coding and non-coding RNA-binding proteins (RBPs) in the Leishmania spp. Towards this we applied the recently reported orthogonal organic phase separation (OOPS) method in combination with tandem mass tag (TMT) labelling-based quantitative proteomic mass spectrometry (MS) and report herein the most comprehensive identification of RBPs in Leishmania mexicana (L. mexicana) parasites. This study identified novel RNA binding property of thousands of L. mexicana proteins, significantly expanding the RBP landscape of the parasite. Furthermore, we showed that the classical Hsp90 inhibitor tanespimycin differentially regulates the RNA-binding property of hundreds of L. mexicana RBPs, shedding light into hitherto unknown large-scale downstream molecular effects of the small molecule inhibitor in the parasite. |
HostingRepository | PRIDE |
AnnounceDate | 2022-01-14 |
AnnouncementXML | Submission_2022-01-14_00:14:05.089.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Kalesh Karunakaran |
SpeciesList | scientific name: Leishmania mexicana MHOM/GT/2001/U1103; NCBI TaxID: 929439; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-08-24 03:11:21 | ID requested | |
⏵ 1 | 2022-01-14 00:14:05 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Leishmania, RNA-binding proteins |
Contact List
Kalesh Karunakaran |
contact affiliation | Department of Chemistry, Durham University, South Road, Durham, UK |
contact email | kalesh.karunakaran@durham.ac.uk |
lab head | |
Kalesh Karunakaran |
contact affiliation | Durham University |
contact email | kalesh.karunakaran@durham.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD028092
- Label: PRIDE project
- Name: Transcriptome-wide identification of coding and noncoding RNA-binding proteins defines the extended RNA interactome of Leishmania mexicana