PXD028092

PXD028092 is an original dataset announced via ProteomeXchange.

Title	Transcriptome-wide identification of coding and noncoding RNA-binding proteins defines the extended RNA interactome of Leishmania mexicana
Description	Non-poly(A) RNA molecules including noncoding RNAs (ncRNAs) comprise the major portion of the total transcribed molecules in the cell. In addition to the mRNAs the ncRNAs also function as ribonucleoprotein particles (RNPs) and carry out biological functions including synthesis of new proteins, RNA processing, genome remodelling and regulation of transcription. We therefore envisaged a comprehensive transcriptome-wide identification of coding and non-coding RNA-binding proteins (RBPs) in the Leishmania spp. Towards this we applied the recently reported orthogonal organic phase separation (OOPS) method in combination with tandem mass tag (TMT) labelling-based quantitative proteomic mass spectrometry (MS) and report herein the most comprehensive identification of RBPs in Leishmania mexicana (L. mexicana) parasites. This study identified novel RNA binding property of thousands of L. mexicana proteins, significantly expanding the RBP landscape of the parasite. Furthermore, we showed that the classical Hsp90 inhibitor tanespimycin differentially regulates the RNA-binding property of hundreds of L. mexicana RBPs, shedding light into hitherto unknown large-scale downstream molecular effects of the small molecule inhibitor in the parasite.
HostingRepository	PRIDE
AnnounceDate	2022-01-14
AnnouncementXML	Submission_2022-01-14_00:14:05.089.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kalesh Karunakaran
SpeciesList	scientific name: Leishmania mexicana MHOM/GT/2001/U1103; NCBI TaxID: 929439;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-24 03:11:21	ID requested
⏵ 1	2022-01-14 00:14:05	announced

Dataset with its publication pending

submitter keyword: Leishmania, RNA-binding proteins

Kalesh Karunakaran
contact affiliation	Department of Chemistry, Durham University, South Road, Durham, UK
contact email	kalesh.karunakaran@durham.ac.uk
lab head
Kalesh Karunakaran
contact affiliation	Durham University
contact email	kalesh.karunakaran@durham.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028092
     1. Label: PRIDE project
     2. Name: Transcriptome-wide identification of coding and noncoding RNA-binding proteins defines the extended RNA interactome of Leishmania mexicana