PXD027941-1

PXD027941 is an original dataset announced via ProteomeXchange.

Title	Spatial proteomics reveals profound subcellular reorganization in human keratinocytes exposed to UVA light
Description	The effects of UV light on the skin have been extensively investigated. However, systematic information about how exposure to UVA light, the least energetic but the most abundant UV radiation reaching the Earth, shapes the subcellular organization of proteins is lacking. Using subcellular fractionation, mass-spectrometry-based proteomics, machine learning algorithms, immunofluorescence, and functional assays, we mapped the subcellular reorganization of the proteome of human keratinocytes in response to UVA light. Our workflow quantified and assigned subcellular localization and redistribution patterns for over 3000 proteins, of which about 600 were found to redistribute upon UVA exposure. Reorganization of the proteome affected modulators of signaling pathways, cellular metabolism and DNA damage response. Strikingly, mitochondria were identified as the main target of UVA-induced stress. Further investigation demonstrated that UVA induces mitochondrial fragmentation, up-regulates redox-responsive proteins and attenuates respiratory rates. These observations emphasize the role of this radiation as a potent metabolic stressor in the skin.
HostingRepository	PRIDE
AnnounceDate	2022-03-06
AnnouncementXML	Submission_2022-03-06_14:23:19.011.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD027941
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Hellen Valerio
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-16 02:14:39	ID requested
⏵ 1	2022-03-06 14:23:19	announced

Dataset with its publication pending

submitter keyword: spatial proteomics, keratinocytes, UVA light

Paolo Di Mascio
contact affiliation	Institute of Chemistry, University of São Paulo
contact email	pdmascio@iq.usp.br
lab head
Hellen Valerio
contact affiliation	Universidade de São Paulo
contact email	hellen.valerio@usp.br
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD027941

PRIDE project URI

• PRIDE
  1. PXD027941
     1. Label: PRIDE project
     2. Name: Spatial proteomics reveals profound subcellular reorganization in human keratinocytes exposed to UVA light