PXD027874

PXD027874 is an original dataset announced via ProteomeXchange.

Title	Vibrio natriegens Systems & Synthetic Biology: Proteome Profile (Temperature & Salinity)
Description	Vibrio natriegens is a rapidly growing salt marsh bacterium that is being developed as a synthetic biology chassis. We characterized its physiological response to different salinities and temperatures in order to optimize culturing conditions and understand its adaptations to a salt marsh environment. Using metabolomics, transcriptomics, and proteomics we determined what pathways respond to these environmental parameters. We found that organic osmolyte synthesis and membrane transporters were most responsive to changes in salinity. The primary osmolytes were glutamate, glutamine, and ectoine, responding to salinity across temperature treatments. However, when media was supplemented with choline, glycine betaine seemed to mostly replace ectoine. These results provide a baseline dataset of metabolic activity under a variety of conditions that will inform decisions made about culturing and genome engineering for future applications.
HostingRepository	PRIDE
AnnounceDate	2021-09-15
AnnouncementXML	Submission_2021-09-15_13:04:14.588.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Judson Hervey
SpeciesList	scientific name: Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759; NCBI TaxID: 1219067;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-11 22:13:49	ID requested
⏵ 1	2021-09-15 13:04:14	announced

Dataset with its publication pending

submitter keyword: Synthetic Biology, nanoLC-MS/MS, proteome, proteomics, Vibrio, Vibrio natriegens

J Hervey
contact affiliation	CBMSE
contact email	hervey.judson@gmail.com
lab head
Judson Hervey
contact affiliation	Naval Research Laboratory
contact email	judson.hervey@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD027874

PRIDE project URI

• PRIDE
  1. PXD027874
     1. Label: PRIDE project
     2. Name: Vibrio natriegens Systems & Synthetic Biology: Proteome Profile (Temperature & Salinity)