PXD027831-1
PXD027831 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Retention time as an extra dimension for proteome-wide cross-link identification |
Description | Chemical crosslinking methods have not been successfully applied to protein-protein interaction discovery mainly due to the computation complexity (O (n2)) issue. In a previous report, we proposed a decision tree searching strategy which can reduce complexity by orders of magnitude. In this study, we found that the mono-linked peptides carry out the information of the retention time of the cross-linked pairs, therefore the retention time of cross-linked peptide pairs can be predicted accurately. By utilizing the obtained retention time information, the false positive rate can be reduced by around 86% with a sensitivity loss of 12%. The method was successfully applied to the identification of inter cross-links obtained from E. coli cell lysate crosslinked by a newly synthesized enrichable crosslinker. |
HostingRepository | iProX |
AnnounceDate | 2021-08-06 |
AnnouncementXML | Submission_2021-08-09_21:54:13.431.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Wei Zhu |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion; Orbitrap Fusion ETD |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2021-08-09 21:53:30 | ID requested | |
⏵ 1 | 2021-08-09 21:54:13 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: retention time, crosslink-MS |
Contact List
Wei Zhu | |
---|---|
contact affiliation | ShanghaiTech University |
contact email | zhuwei@shanghiatech.edu.cn |
lab head | |
Wei Zhu | |
contact affiliation | Shanghaitech University |
contact email | zhuwei@shanghaitech.edu.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |