PXD027831

PXD027831 is an original dataset announced via ProteomeXchange.

Title	Retention time as an extra dimension for proteome-wide cross-link identification
Description	Chemical crosslinking methods have not been successfully applied to protein-protein interaction discovery mainly due to the computation complexity (O (n2)) issue. In a previous report, we proposed a decision tree searching strategy which can reduce complexity by orders of magnitude. In this study, we found that the mono-linked peptides carry out the information of the retention time of the cross-linked pairs, therefore the retention time of cross-linked peptide pairs can be predicted accurately. By utilizing the obtained retention time information, the false positive rate can be reduced by around 86% with a sensitivity loss of 12%. The method was successfully applied to the identification of inter cross-links obtained from E. coli cell lysate crosslinked by a newly synthesized enrichable crosslinker.
HostingRepository	iProX
AnnounceDate	2021-08-06
AnnouncementXML	Submission_2021-08-09_21:54:13.431.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wei Zhu
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion; Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-09 21:53:30	ID requested
⏵ 1	2021-08-09 21:54:13	announced

Dataset with its publication pending

submitter keyword: retention time, crosslink-MS

Wei Zhu
contact affiliation	ShanghaiTech University
contact email	zhuwei@shanghiatech.edu.cn
lab head
Wei Zhu
contact affiliation	Shanghaitech University
contact email	zhuwei@shanghaitech.edu.cn
dataset submitter

iProX dataset URI