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PXD027757-1

PXD027757 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMitochondrial interactome quantitation reveals structural changes in metabolic machinery in failing murine heart
DescriptionAdvancements of cross-linking mass spectrometry (XL-MS) for structural analysis of proteins bridges the gap between purified systems and native tissue environments. Here, we utilize isobaric quantitative protein interaction reporter technology (iqPIR) to further extend XL-MS to the first system-wide comparative study of mitochondrial proteins from healthy and diseased murine hearts. The failing heart interactome includes 544 statistically significant cross-linked peptide pairs altered in disease condition. Structural insight into ketone oxidation metabolons, OXPHOS machinery, and nucleotide transporter hybrid-conformations, support mitochondrial remodeling in failing heart while bringing forth new hypotheses for pathological mechanisms. Application of quantitative cross-linking technology in live tissue provides molecular-level insight to complex biological systems difficult to model in cell culture, thus providing a valuable resource for study of human diseases.
HostingRepositoryPRIDE
AnnounceDate2022-08-02
AnnouncementXMLSubmission_2022-08-02_03:36:35.753.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterXiaoting Tang
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-08-05 10:01:28ID requested
12022-08-02 03:36:36announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: quantitative cross-linking
heart failure
cardiac hypertrophy
mitochondria
protein interactions
interactome
mass spectrometry
systems structural biologyquantitative cross-linking
heart failure
cardiac hypertrophy
mitochondria
protein interactions
interactome
mass spectrometry
systems structural biology
Contact List
James Edward Bruce
contact affiliationDepartment of Genome Sciences, University of Washington
contact emailjimbruce@uw.edu
lab head
Xiaoting Tang
contact affiliationUniversity of Washington
contact emailxiaoting@uw.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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