PXD027743 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantitative fragmentomics to map affinities of interactomes |
Description | ~500,000 protein interactions have been identified in human but most binding affinities remain unknown, limiting quantitative investigation of interactome-function relationships. This gap can be filled by systematically measuring the affinities of minimal interacting protein fragments, the building blocks of interactomes. As a proof of principle, we experimentally measured 65,000 affinities involving 266 human PDZ domains (practically the complete "PDZome"), 424 human PDZ-Binding Motifs (~10% of the "PBMome") and 24 viral motifs. We determined binding constants for ~18,000 interactions that passed the detection threshold. Independent AP-MS experiments and database surveys demonstrated the strong agreement of quantitative fragmentomics with full-length protein interactomics. We evidence hot spots for viral interference, which allow a viral PBM, such as that of HPV-E6 oncoprotein, to impact the cell proteome way beyond its immediate binders. We propose to distinguish interactomes and complexomes, viewed as intrinsic and extrinsic properties linked by the law of mass action. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:32:04.220.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Luc Negroni |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-08-05 09:59:53 | ID requested | |
1 | 2022-10-14 09:18:20 | announced | |
⏵ 2 | 2023-11-14 08:32:05 | announced | 2023-11-14: Updated project metadata. |
Publication List
Gogl G, Zambo B, Kostmann C, Cousido-Siah A, Morlet B, Durbesson F, Negroni L, Eberling P, Jan, é P, Nomin, é Y, Zeke A, Ø, stergaard S, Monsellier É, Vincentelli R, Trav, é G, Quantitative fragmentomics allow affinity mapping of interactomes. Nat Commun, 13(1):5472(2022) [pubmed] |
Keyword List
submitter keyword: complexome,PDZ, domain-motif interaction, interactome |
Contact List
Gergo Gogl |
contact affiliation | Institut de Génétique et de Biologie Moléculaire et Cellulaire, 67404 Illkirch CEDEX, France |
contact email | goglg@igbmc.fr |
lab head | |
Luc Negroni |
contact affiliation | CNRS |
contact email | luc.negroni@igbmc.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD027743
- Label: PRIDE project
- Name: Quantitative fragmentomics to map affinities of interactomes