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PXD027678

PXD027678 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteome and peptidome analyses of mouse caecal contents and Paraprevotella clara culture supernatants
DescriptionCaecal contents from three specific pathogen-free (SPF) mice, and three germ-free (GF) mice were analyzed by DIA-MS (SWATH-MS) using TripleTOF 5600+ (SCIEX). Paraprevotella clara (P. clara) culture supernatants nontreated and treated tunicamycin or 2-fluro-L-fucose (2F-Fuc) were analyzed by DIA-MS using Q Exactive HF-X (Thermo Fisher Scientific). In addition, GF mouse caecal contents were incubated with P. clara or medium control. Supernatant samples were collected at the indicated time points and subjected to peptidome analysis using Q Exactive HF-X (Thermo Fisher Scientific).
HostingRepositoryjPOST
AnnounceDate2022-06-29
AnnouncementXMLSubmission_2022-09-19_18:17:20.731.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYusuke Kawashima
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Paraprevotella clara; NCBI TaxID: 454154;
ModificationListS-carboxamidomethyl-L-cysteine; unknown modification; L-methionine sulfoxide
InstrumentTripleTOF 5600; Q Exactive HF-X
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-07-31 21:37:17ID requested
12022-06-29 02:06:21announced
22022-09-18 03:18:48announced2022-09-18: Updated FTP location.
32022-09-19 18:17:21announced2022-09-20: Updated PubMed.
Publication List
Li Y, Watanabe E, Kawashima Y, Plichta DR, Wang Z, Ujike M, Ang QY, Wu R, Furuichi M, Takeshita K, Yoshida K, Nishiyama K, Kearney SM, Suda W, Hattori M, Sasajima S, Matsunaga T, Zhang X, Watanabe K, Fujishiro J, Norman JM, Olle B, Matsuyama S, Namkoong H, Uwamino Y, Ishii M, Fukunaga K, Hasegawa N, Ohara O, Xavier RJ, Atarashi K, Honda K, Identification of trypsin-degrading commensals in the large intestine. Nature, 609(7927):582-589(2022) [pubmed]
Keyword List
submitter keyword: caecal content, specific pathogen-free, germ-free, Paraprevotella clara, DIA-MS, peptidomics
Contact List
Kenya Honda
lab head
Yusuke Kawashima
contact affiliationKazusa DNA Research Institute
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001279/