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PXD027534-1

PXD027534 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteome of mouse striatal dopamine synaptosomes purified by fluorescence sorting
DescriptionDopamine transmission is a monoaminergic system involved in reward processing and motor control. Volume transmission is thought to be the main mechanism by which monoamines modulate effector transmission though synaptic structures are scarcely described. In the present work we aimed to unravel the cellular and molecular synaptome of single projection pathways (Zhu F, Cizeron M, Qiu Z, Benavides-Piccione R, Kopanitsa MV, Skene NG, Koniaris B, DeFelipe J, Fransén E, Komiyama NH & Grant SGN (2018) Architecture of the Mouse Brain Synaptome. Neuron 99: 781–799.e10). To that end, we established a workflow combining fluorescence tracing of the dopaminergic pathway, fluorescence activated synaptosome sorting and mass spectrometry-based proteomics. With this approach we provide the first ex-vivo model to thoroughly analyse the cellular and molecular organisation of dopaminergic synapses from mouse striatum.
HostingRepositoryPRIDE
AnnounceDate2022-06-09
AnnouncementXMLSubmission_2022-06-09_04:17:39.883.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterStephane Claverol
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListphosphorylated residue; monohydroxylated residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-07-25 14:33:21ID requested
12022-06-09 04:17:40announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Dopamine, synapse, synaptosome, FASS, mouse, striatum, proteome
Contact List
Stephane Claverol
contact affiliationPlateforme Proteome, Univ.Bordeaux
contact emailstephane.claverol@u-bordeaux.fr
lab head
Stephane Claverol
contact affiliationPlateforme Proteome
contact emailstephane.claverol@u-bordeaux.fr
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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