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PXD027452-1

PXD027452 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAnnexin A1 is a polarity cue that directs planar mitotic spindle orientation during mammalian epithelial morphogenesis
DescriptionOriented cell divisions are critical for the formation and maintenance of structured epithelia. Proper mitotic spindle orientation relies on polarised anchoring of force generators to the cell cortex by the evolutionarily conserved Gαi-LGN-NuMA complex. However, the polarity cues that control cortical patterning of this ternary complex remain largely unknown in mammalian epithelia. Here we identify the membrane-associated protein Annexin A1 (ANXA1) as a novel interactor of LGN in mammary epithelial cells. ANXA1 acts independently of Gαi to instruct the accumulation of LGN and NuMA at the lateral cortex to ensure cortical anchoring of Dynein-Dynactin and astral microtubules and thereby planar alignment of the mitotic spindle. Loss of ANXA1 randomises mitotic spindle orientation, which in turn disrupts epithelial architecture and lumen formation in three-dimensional (3D) primary mammary organoids. Our findings establish ANXA1 as an upstream cortical cue that regulates LGN to direct planar cell divisions during mammalian epithelial morphogenesis.
HostingRepositoryPRIDE
AnnounceDate2022-12-02
AnnouncementXMLSubmission_2022-12-02_03:52:06.238.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPaulSkipp
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-07-20 22:26:38ID requested
12022-12-02 03:52:06announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: mammary,Proteomics, Annexin A1
Contact List
SalahElias
contact affiliationBiological Sciences University of Southampton
contact emails.k.elias@soton.ac.uk
lab head
PaulSkipp
contact affiliationCentre for Proteomic Research, University of Southampton
contact emailpjss@soton.ac.uk
dataset submitter
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Dataset FTP location
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