PXD027420 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Retroelement Ty1 Integrase interactome identified using tandem chromatin affinity purification ( TChAP) after in vivo crosslink in yeast Saccharomyces cerevisiae |
Description | To identify yeast proteins associated with Ty1 integrase (IN) that could regulate Ty1 replication, we co-purified IN partners using the tandem chromatin affinity purification procedure after in vivo cross-link (TChAP), which we developed previously (Nguyen et al. 2014). We first identified RNA Pol I and Pol III complexes and also a small subset of additional evolutionary conserved complexes, including PAF1 (Polymerase-Associated Factor 1),FACT (FAcilitates Chromatin Transcription), the proteasome and the CK2 kinase. We next confirmed that CK2 interacts with Ty1 integrase in vivo and repress Ty1 retromobility. We showed that Ty1 IN is a substrate of CK2 in vitro and identified 12 phosphorylated residues. In vivo approaches showed that only part of the protein was phosphorylated in the cells and did not demonstrate any direct evidence between Ty1 IN phosphorylation and retromobility inhibition. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:52:31.237.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD027420 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Agata Malinowska |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-07-19 08:54:45 | ID requested | |
1 | 2022-11-17 05:19:02 | announced | |
⏵ 2 | 2023-11-14 08:52:31 | announced | 2023-11-14: Updated project metadata. |
Publication List
Keyword List
submitter keyword: RNA Polymerase III, yeast, CK2, integrase,Ty1 |
Contact List
Joël Acker |
contact affiliation | Integrative Biology and Molecular Genetics Unit (SBIGeM) |
contact email | Joel.ACKER@cea.fr |
lab head | |
Agata Malinowska |
contact affiliation | IBB PAS |
contact email | esme@ibb.waw.pl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD027420
- Label: PRIDE project
- Name: Retroelement Ty1 Integrase interactome identified using tandem chromatin affinity purification ( TChAP) after in vivo crosslink in yeast Saccharomyces cerevisiae