PXD027401-1

PXD027401 is an original dataset announced via ProteomeXchange.

Title	Hydrogen Deuterium Exchange of PDK1
Description	This project consisted of three HDX-MS experiments. First, we compared the dimeric PDK1(SKD-PIF) to monomeric PDK1(SKD) and mapped the differences in deuterium incorporation onto the dimer model. We then compared the deuterium incorporation kinetics for the kinase (PDK1(SKD)) and PH (PDK1(PH) domains of PDK1 with full-length PDK1 (PDK1(FL)) in pairwise experiments.
HostingRepository	PRIDE
AnnounceDate	2022-05-20
AnnouncementXML	Submission_2022-05-20_03:20:56.258.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	John Burke
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	impact HD

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-19 02:36:52	ID requested
⏵ 1	2022-05-20 03:20:57	announced

Levina A, Fleming KD, Burke JE, Leonard TA, Activation of the essential kinase PDK1 by phosphoinositide-driven trans-autophosphorylation. Nat Commun, 13(1):1874(2022) [pubmed]

submitter keyword: HDX-MS PDK1 HDX

John Burke
contact affiliation	University of Victoria
contact email	jeburke@uvic.ca
lab head
John Burke
contact affiliation	University of Victoria
contact email	jeburke@uvic.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD027401

PRIDE project URI

• PRIDE
  1. PXD027401
     1. Label: PRIDE project
     2. Name: Hydrogen Deuterium Exchange of PDK1