PXD027380

PXD027380 is an original dataset announced via ProteomeXchange.

Title	Hippocampal subfields proteome analysis and comparation in Alzheimer’s disease providing new sight on glia function
Description	The hippocampus and entorhinal cortex are the earliest areas affected by AD pathology, which are related to early memory loss in patients. The hippocampus subfields are heterogeneous, with different order and degree affect by AD pathology. Studying the molecular changes may explain the difference, and may provide new therapeutic ideas for AD. In this study, specimens from the Chinese human brain banking were used to compare the proteome changes in the hippocampal subfields and the EC area affected by AD. The region-specific differential expressed proteins (associated with oligodendrocytes and myelin sheath) and co-variant proteins, also considered as constitute proteins (associated with astrocytes) were identified. The IHC results verified the specific loss of myelin in CA1, CA3 and EC areas, and find that protective S100A10-positive astrocytes number was increased in all hippocampal subfields and EC area. Further dual immunofluorescence results find that S100A10-positive astrocytes phagocyte apoptotic neuron debris. A hypothesis was put forward based on this, that S100A10-positive astrocytes protect neurons probably through engulfing apoptotic neurons. This study conducts a comprehensive proteomic analysis on the hippocampus subregion for the first time, highlighting the glia role in AD, and providing more information on the pathogenesis and therapy of AD.
HostingRepository	iProX
AnnounceDate	2021-07-16
AnnouncementXML	Submission_2022-12-19_18:07:51.916.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yanpan Gao
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	TMT6plex reporter fragment; phosphorylated residue; deaminated residue
Instrument	Orbitrap Fusion Lumos; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-18 20:56:19	ID requested
1	2021-07-18 20:58:21	announced
⏵ 2	2022-12-19 18:07:52	announced	2022-12-20: Update information publication.

Gao Y, Liu J, Wang J, Liu Y, Zeng LH, Ge W, Ma C, Proteomic analysis of human hippocampal subfields provides new insights into the pathogenesis of Alzheimer's disease and the role of glial cells. Brain Pathol, 32(4):e13047(2022) [pubmed]

submitter keyword: Alzheimer’s disease, hippocampal subfields, entorhinal cortex, proteomics, glia

Wei Ge
contact affiliation	Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College
contact email	wei.ge@chem.ox.ac.uk
lab head
Yanpan Gao
contact affiliation	Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College
contact email	gaoyanpan@gmail.com
dataset submitter

iProX dataset URI