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PXD027318-1

PXD027318 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSenescence Biomarker Discovery by LC-MS-based Proteomics
DescriptionSenescence is a permanent cell cycle arrest that occurs in response to cellular stress. Because senescent cells promote age-related disease, there has been considerable interest in defining the proteomic alterations in senescent cells. Because senescence differs greatly depending on cell type and senescence inducer, continued progress in the characterization of senescent cells is needed. Here, we analyzed primary human mammary epithelial cells (HMECs), a model system for aging, using mass spectrometry-based proteomics. By integrating data from replicative senescence, immortalization by telomerase reactivation, and drug-induced senescence, we identified a robust proteomic signature of HMEC senescence consisting of 77 upregulated and 36 downregulated proteins. This approach identified known biomarkers, such as downregulation of the nuclear lamina protein lamin-B1 (LMNB1), and novel upregulated proteins including the β-galactoside-binding protein galectin-7 (LGALS7). Gene ontology enrichment analysis demonstrated that senescent HMECs upregulated lysosomal proteins and downregulated RNA metabolic processes. We additionally integrated our proteomic signature of senescence with transcriptomic data from senescent HMECs to demonstrate that our proteomic signature can discriminate proliferating and senescent HMECs even at the transcriptional level. Taken together, our results demonstrate the power of proteomics to identify cell type-specific signatures of senescence and advance the understanding of senescence in primary HMECs.
HostingRepositoryPRIDE
AnnounceDate2021-09-28
AnnouncementXMLSubmission_2021-09-28_09:17:11.451.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAlireza Delfarah
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-07-14 22:12:53ID requested
12021-09-28 09:17:11announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: senescence, aging, proteomics, mammary epithelial cells
Contact List
Nicholas Alexander Graham
contact affiliationMork Family Department of Chemical Engineering and Materials Science, University of Southern California, USA
contact emailnagraham@usc.edu
lab head
Alireza Delfarah
contact affiliationUniversity of Southern California
contact emaildelfarah@usc.edu
dataset submitter
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Dataset FTP location
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