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PXD027301-1

PXD027301 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA new mass spectral library for high-coverage and reproducible analysis of the Plasmodium falciparum-infected red blood cell proteome
DescriptionBackground: Plasmodium falciparum causes the majority of malaria mortality worldwide, and the disease occurs during the asexual red blood cell (RBC) stage of infection. In the absence of an effective and available vaccine, and with increasing drug resistance, asexual RBC stage parasites are an important research focus. In recent years, mass spectrometry-based proteomics using Data Dependent Acquisition (DDA) has been extensively used to understand the biochemical processes within the parasite. However, DDA is problematic for the detection of low abundance proteins, protein coverage, and has poor run-to-run reproducibility. Results: Here, we present a comprehensive P. falciparum-infected RBC (iRBC) spectral library to measure the abundance of 44,449 peptides from 3,113 P. falciparum and 1,617 RBC proteins using a Data Independent Acquisition (DIA) approach. The spectral library includes proteins expressed in the three morphologically distinct RBC stages (ring, trophozoite, schizont), the RBC compartment of trophozoite-iRBCs, and the cytosolic fractions from uninfected RBCs (uRBC). This spectral library contains 87% of P. falciparum proteins with previously blood-stage protein-level evidence as well as 692 previously unidentified proteins. The P. falciparum spectral library was successfully applied to generate semi-quantitative proteomics datasets that characterise the three distinct asexual parasite stages in RBCs, and compare artemisinin resistant (Cam3.IIR539T) and sensitive (Cam3.IIrev) parasites. Conclusion: A reproducible, high-coverage proteomics spectral library and analysis method has been generated for investigating sets of proteins expressed in the iRBC stage of P. falciparum malaria. This will provide a foundation for an improved understanding of parasite biology, pathogenesis, drug mechanisms and vaccine candidate discovery for malaria.
HostingRepositoryPRIDE
AnnounceDate2021-11-15
AnnouncementXMLSubmission_2021-11-15_04:04:36.347.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterGhizal Siddiqui
SpeciesList scientific name: Plasmodium falciparum; NCBI TaxID: 5833; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-07-14 07:28:53ID requested
12021-11-15 04:04:36announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Plasmodium falciparum, malaria, spectral library, DIA
Contact List
Darren Creek
contact affiliationMonash Institute of Pharmaceutical Sciences, Monash University, Melbourne, Australia
contact emaildarren.creek@monash.edu
lab head
Ghizal Siddiqui
contact affiliationMonash University
contact emailghizal.siddiqui@monash.edu
dataset submitter
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Dataset FTP location
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