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PXD027195-1

PXD027195 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleConserved exchange of paralog proteins during neuronal differentiation
DescriptionGene duplication enables the emergence of new functions by lowering the general evolutionary pressure. Previous studies have highlighted the role of specific paralog genes during cell differentiation, e.g., in chromatin remodeling complexes. It remains unexplored whether similar mechanisms extend to other biological functions and whether the regulation of paralog genes is conserved across species. Here, we analyze the expression of paralogs across human tissues, during development and neuronal differentiation in fish, rodents and humans. While ~80% of paralog genes are co-regulated, a subset of paralogs shows divergent expression profiles, contributing to variability of protein complexes. We identify 78 substitutions of paralog eggNOG pairs that occur during neuronal differentiation and are conserved across species. Among these, we highlight a substitution between the paralogs Sec23a and Sec23b subunits of the COPII complex. Altering the ratio between these two genes via silencing-RNA knockdown was able to influence neuronal differentiation in different ways. We propose that remodeling of the vesicular transport system via paralog substitutions is an evolutionary conserved mechanism enabling neuronal differentiation.
HostingRepositoryPRIDE
AnnounceDate2022-03-07
AnnouncementXMLSubmission_2022-03-07_10:14:49.406.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterEmilio Cirri
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListcarbamoylated residue; acetylated residue; monohydroxylated residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-07-08 09:10:34ID requested
12022-03-07 10:14:49announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: paralogs, neuronal differentiation, protein complexes, mouse
Contact List
Alessandro Ori
contact affiliationLeibniz Institute on Aging - Fritz Lipmann Institute (FLI) Beutenbergstraße 11 07745 Jena, Germany
contact emailAlessandro.Ori@leibniz-fli.de
lab head
Emilio Cirri
contact affiliationLeibniz Institute on Ageing Fritz Lipmann Institute Jena
contact emailemilio.cirri@fli-leibniz.de
dataset submitter
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Dataset FTP location
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