PXD027168-1

PXD027168 is an original dataset announced via ProteomeXchange.

Title	Affinity Purification Mass Spectrometry Analysis of Mutant and Wildtype SARS-CoV-2 Nucleocapsid Protein
Description	The COVID-19 pandemic is arguably the most important global threat to humankind that the world has seen in a century. SARS-CoV-2 has now reached 196 countries across all continents with over 159 million confirmed cases with 3.3 million deaths todate. Currently, our knowledge on how and why SARS-CoV-2 causes severe COVID-19 is continually evolving. Here, we identified three consecutive SNPs (G28881A, G28882A, G28883C) resulting in the Nucleocapsid (N) protein amino acid mutations R203K and G204R (simply termed KR mutation) by large-scale SARS-CoV-2 genome sequencing from clinical swab samples of patients. The nucleocapsid (N) protein of SARS-CoV-2, the highly abundant structure protein within the infected cells, serves multiple functions during viral infection, which besides RNA binding, playing essential roles in viral transcription, replication, and translation. We investigated the impact of this KR mutation on the functional properties of N protein in terms of host protein interaction and phosphorylation status by mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2021-12-02
AnnouncementXML	Submission_2021-12-02_11:34:30.368.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Muhammad Shuaib
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-07 09:24:41	ID requested
⏵ 1	2021-12-02 11:34:30	announced
2	2023-04-11 06:28:04	announced	2023-04-11: Updated project metadata.

Dataset with its publication pending

submitter keyword: COVID-19, SARS-CoV-2, Nucleocapsid Protein, Data dependent acqusition (DDA), LC-MS/MS

Muhammad Shuaib
contact affiliation	King Abdullah University of Science and Technology, Pathogen Genomic Group, Thuwal 23500-6900, Kingdom of Saudi Arabia
contact email	muhammad.shuaib@kaust.edu.sa
lab head
Muhammad Shuaib
contact affiliation	King Abdullah University of Science and Technology
contact email	muhammad.shuaib@kaust.edu.sa
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/12/PXD027168

PRIDE project URI

• PRIDE
  1. PXD027168
     1. Label: PRIDE project
     2. Name: Affinity Purification Mass Spectrometry Analysis of Mutant and Wildtype SARS-CoV-2 Nucleocapsid Protein