PXD027158-1

PXD027158 is an original dataset announced via ProteomeXchange.

Title	CLUH interactome reveals its association to SPAG5 and its proximity to the translation of mitochondrial proteins
Description	Mitochondria require thousands of proteins to fulfil their essential function in energy production and other fundamental biological processes. These proteins are mostly encoded by the nuclear genome, translated in the cytoplasm before being imported into the organelle. RNA binding proteins (RBPs) are central players in the regulation of this process by affecting mRNA translation, stability or localization. CLUH is an RBP recognizing specifically mRNAs coding for mitochondrial proteins, but its precise molecular function and interacting partners remain undiscovered in mammals. Here we reveal for the first time CLUH interactome in mammalian cells. Using both co-IP and BioID proximity-labeling approaches, we identify novel molecular partners interacting stably or transiently with CLUH in HCT116 cells and mouse embryonic stem cells. We reveal a stable RNA-independent interaction of CLUH with itself and with SPAG5 in cytosolic granular structures. More importantly, we uncover an unexpected proximity of CLUH to mitochondrial proteins and its cognate mRNAs in the cytosol. Additionally, our data highlights the importance of CLUH TPR domain for its interactions with both proteins and mRNAs. Overall, through the analysis of CLUH interactome, our study sheds a new light on CLUH molecular function by highlighting its association to the translation and subcellular localization of some mRNAs coding for mitochondrial proteins.
HostingRepository	PRIDE
AnnounceDate	2021-12-13
AnnouncementXML	Submission_2021-12-13_05:59:50.984.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD027158
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Johana Chicher
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-07 07:04:13	ID requested
⏵ 1	2021-12-13 05:59:51	announced

Dataset with its publication pending

submitter keyword: CLUH, mitochondrial proteins, RNA binding protein, BioID, TurboID

Richard Patryk Ngondo
contact affiliation	Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg, Strasbourg, France
contact email	patryk.ngondo@unistra.fr
lab head
Johana Chicher
contact affiliation	Université de Strasbourg, Plateforme Protéomique Strasbourg-Esplanade, Centre National de la Recherche Scientifique
contact email	j.chicher@ibmc-cnrs.unistra.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027158
     1. Label: PRIDE project
     2. Name: CLUH interactome reveals its association to SPAG5 and its proximity to the translation of mitochondrial proteins