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PXD027158

PXD027158 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCLUH interactome reveals its association to SPAG5 and its proximity to the translation of mitochondrial proteins
DescriptionMitochondria require thousands of proteins to fulfil their essential function in energy production and other fundamental biological processes. These proteins are mostly encoded by the nuclear genome, translated in the cytoplasm before being imported into the organelle. RNA binding proteins (RBPs) are central players in the regulation of this process by affecting mRNA translation, stability or localization. CLUH is an RBP recognizing specifically mRNAs coding for mitochondrial proteins, but its precise molecular function and interacting partners remain undiscovered in mammals. Here we reveal for the first time CLUH interactome in mammalian cells. Using both co-IP and BioID proximity-labeling approaches, we identify novel molecular partners interacting stably or transiently with CLUH in HCT116 cells and mouse embryonic stem cells. We reveal a stable RNA-independent interaction of CLUH with itself and with SPAG5 in cytosolic granular structures. More importantly, we uncover an unexpected proximity of CLUH to mitochondrial proteins and its cognate mRNAs in the cytosol. Additionally, our data highlights the importance of CLUH TPR domain for its interactions with both proteins and mRNAs. Overall, through the analysis of CLUH interactome, our study sheds a new light on CLUH molecular function by highlighting its association to the translation and subcellular localization of some mRNAs coding for mitochondrial proteins.
HostingRepositoryPRIDE
AnnounceDate2021-12-13
AnnouncementXMLSubmission_2021-12-13_05:59:50.984.xml
DigitalObjectIdentifierhttp://dx.doi.org/10.6019/PXD027158
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterJohana Chicher
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-07-07 07:04:13ID requested
12021-12-13 05:59:51announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: CLUH, mitochondrial proteins, RNA binding protein, BioID, TurboID
Contact List
Richard Patryk Ngondo
contact affiliationInstitut de biologie moléculaire des plantes, CNRS, Université de Strasbourg, Strasbourg, France
contact emailpatryk.ngondo@unistra.fr
lab head
Johana Chicher
contact affiliationUniversité de Strasbourg, Plateforme Protéomique Strasbourg-Esplanade, Centre National de la Recherche Scientifique
contact emailj.chicher@ibmc-cnrs.unistra.fr
dataset submitter
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