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PXD027098-2

PXD027098 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePost-translational responses to elevated c-di-GMP levels in Pseudomonas aeruginosa
DescriptionTo analyze the impact of elevated c-di-GMP concentrations in P. aeruginosa, we expressed pleD* on an inducible vector (pHERD20T) in the PAO1 wild-type strain. PleD is a DGC from Caulobacter cresentusand the pleD* construct variant encodes for a constitutively active enzyme due to four amino acid exchanges (T120N, T214A, P234H, N357Y).We aimed to analyze the cellular consequences of increased c-di-GMP levels in the opportunistic pathogen P. aeruginosa on a global scale. We therefore grew the pleD* harboring PAO1 as well as the empty vector control PAO1 in LB medium, added arabinose (0.2%) to the medium to induce pleD* expression and harvested the cells in exponential growth phase, when the cells exhibited elevated c-di-GMP levels of about two-fold (see manuscript). We are discribing the characteristics of elevated c-di-GMP with a Multi-Omics-Dataset.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:53:15.908.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJulius Ned Witte
SpeciesList scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: 208964;
ModificationListmonohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-07-05 02:06:55ID requested
12022-07-25 03:06:57announced
22023-11-14 08:53:16announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Multi-omics dataset, PleD-overexpression, elevated c-di-GMP levels
Contact List
Susanne Hauessler
contact affiliationInstitute for Molecular Bacteriology, TWINCORE GmbH, Center of Clinical and Experimental Infection Research, a joint venture of the Hannover Medical School and the Helmholtz Center for Infection Research, Hannover, Germany.
contact emailSusanne.haeussler@helmholtz-hzi.de
lab head
Julius Ned Witte
contact affiliationTWINCORE GmbH and Proteomics Core Unit MHH
contact emailjulius.witte@twincore.de
dataset submitter
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Dataset FTP location
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