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PXD027060-1

PXD027060 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLong-lived mitochondrial cristae proteins in mouse heart and brain
DescriptionLong-lived proteins (LLPs) have recently emerged as vital components of intracellular structures whose function is coupled to long-term stability. Mitochondria are multifaceted organelles and their function hinges on the efficient proteome renewal and replacement. Here, using metabolic stable isotope labelling of mice combined with mass spectrometry (MS) based proteomic analysis we demonstrate remarkable longevity for a subset of the mitochondrial proteome. We discovered that mitochondrial LLPs (mt-LLPs) can persist for months in tissues harboring long-lived cells, such as brain and heart. Our analysis revealed an enrichment of mt-LLPs within the inner mitochondrial membrane, specifically in the cristae sub-compartment, and demonstrate that the mitochondrial proteome is not turned over in bulk. Pioneering crosslinking experiments revealed that mt-LLPs are spatially restricted and co-preserved within protein OXPHOS complexes with limited subunit exchange throughout their lifetime. This study provides an explanation for the exceptional mitochondrial protein lifetimes and supports the concept that LLPs provide key structural stability to multiple large and dynamic intracellular structures.
HostingRepositoryMassIVE
AnnounceDate2021-07-02
AnnouncementXMLSubmission_2021-07-02_07:17:19.811.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterJeffrey N. Savas
SpeciesList scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationListCarbamidomethyl
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-07-02 07:13:30ID requested
12021-07-02 07:17:20announced
Publication List
no publication
Keyword List
submitter keyword: mitochondria, cristae, long-lived protein, cross-linking,
Contact List
Jeffrey N. Savas, PhD
contact affiliationDepartment of Neurology Northwestern University
contact emailjeffrey.savas@northwestern.edu
lab head
Jeffrey N. Savas
contact affiliationNorthwestern University
contact emailjeffrey.savas@northwestern.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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