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PXD026930-1

PXD026930 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleImpact of Alanyl-tRNA Synthetase Editing Deficiency in Yeast
DescriptionAminoacyl-tRNA synthetases (aaRSs) are essential enzymes that provide the ribosome with aminoacyl-tRNA substrates for protein synthesis. Mutations in aaRSs lead to various neurological disorders in humans. Many aaRSs utilize editing to prevent error propagation during translation. Editing defects in alanyl-tRNA synthetase (AlaRS) cause neurodegeneration and cardioproteinopathy in mice and is associated with microcephaly in human patients. The cellular impact of AlaRS editing deficiency in eukaryotes remains unclear. Here we use yeast as a model organism to systematically investigate the physiological role of AlaRS editing. Our RNA sequencing and quantitative proteomics analyses reveal that AlaRS editing defects surprisingly activate the general amino acid control pathway and attenuate the heatshock response. We have confirmed these results with reporter and growth assays. In addition, AlaRS editing defects downregulate carbon metabolism and attenuate protein synthesis. Supplying yeast cells with extra carbon source partially rescues the heat sensitivity caused by AlaRS editing deficiency. These findings are in stark contrast with the cellular effects caused by editing deficiency in other aaRSs. Our study therefore highlights the idiosyncratic role of AlaRS editing compared with other aaRSs and provides a model for the physiological impact caused by the lack of AlaRS editing.
HostingRepositoryPRIDE
AnnounceDate2021-08-26
AnnouncementXMLSubmission_2021-08-26_05:20:23.035.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJiqiang Ling
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-06-25 03:54:51ID requested
12021-08-26 05:20:23announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: yeast AlaRS variants, label-free quantitative proteomics
Contact List
Jiqiang Ling
contact affiliationDepartment of Cell Biology and Molecular Genetics University of Maryland
contact emailjling12@umd.edu
lab head
Jiqiang Ling
contact affiliationUniversity of Maryland
contact emailjling12@umd.edu
dataset submitter
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Dataset FTP location
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