PXD026845-1
PXD026845 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Protein interaction studies in human induced neurons indicate convergent biology underlying autism spectrum disorders |
| Description | Autism spectrum disorders (ASD) have been linked to genes with enriched expression in the brain, but it is unclear how these genes converge into cell-type-specific networks. We built a protein-protein interaction network for 13 ASD-associated genes in human excitatory neurons derived from induced pluripotent stem cells (iPSCs). The network contains newly reported interactions and is enriched for genetic and transcriptional perturbations observed in individuals with ASDs. We leveraged the network data to show that the ASD-linked brain-specific isoform of ANK2 is important for its interactions with synaptic proteins and to characterize a PTEN-AKAP8L interaction that influences neuronal growth. The IGF2BP1-3 complex emerged as a convergent point in the network that may regulate a transcriptional circuit of ASD-associated genes. Our findings showcase cell-type-specific interactomes as a framework to complement genetic and transcriptomic data, and illustrate how both individual and convergent interactions can lead to biological insights into ASDs. |
| HostingRepository | MassIVE |
| AnnounceDate | 2023-01-13 |
| AnnouncementXML | Submission_2023-01-13_14:07:55.102.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Lage Lab |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Oxidation; Acetyl; L-cysteine methyl disulfide; Deamidated; Carbamidomethyl; Phospho |
| Instrument | Q Exactive HF-X; TripleTOF 5600+; Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2021-06-21 16:07:33 | ID requested | |
| ⏵ 1 | 2023-01-13 14:07:55 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: autism spectrum disorders, IP-MS, iTRAQ, label-free, induced neurons |
Contact List
| Kasper Lage | |
|---|---|
| contact affiliation | Massachusetts General Hospital |
| contact email | lage.kasper@mgh.harvard.ed |
| lab head | |
| Lage Lab | |
| contact affiliation | Broad Institute of MIT and Harvard |
| contact email | klagelab@gmail.com |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000087676/ |
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