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PXD026843-1

PXD026843 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe E3 ubiquitin ligase RNF115 regulates phagosome maturation and host response to infection
DescriptionPhagocytosis is an essential process for the microbicidal function of professional phagocytes, in which phagosome maturation plays a critical role. Macrophage activation by interferons (IFN) increases their microbicidal activity, but delays phagosomal maturation. Here, we investigated the role of ubiquitylation in phagosome functions. We show that phagosomal proteins are ubiquitylated and that phagosomes contain diverse ubiquitin chain types, including atypical ubiquitin chains. IFN-γ activation of macrophages substantially enhanced phagosomal ubiquitylation of both innate immune response proteins and vesicle trafficking proteins. We identified the E3 ubiquitin ligase RNF115, which is enriched on phagosomes of IFN-γ activated macrophages, as an important regulator of phagosomal maturation. Loss of RNF115 protein or ubiquitin ligase activity facilitated enhanced phagosomal maturation, and increased cytokine responses to Staphylococcus aureus. Our data suggests that both innate immune signalling and phagolysosomal trafficking are controlled through ubiquitylation. In conclusion, we identify RNF115 and ubiquitylation as important regulators of innate immune signalling from the phagosome during bacterial infections.
HostingRepositoryPRIDE
AnnounceDate2022-07-20
AnnouncementXMLSubmission_2022-07-20_05:18:17.813.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMatthias Trost
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListubiquitinylated lysine
InstrumentOrbitrap Fusion Lumos; Orbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-06-21 06:03:20ID requested
12022-07-20 05:18:18announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Phagosome, Macrophage, Ubiquitin, E3-ligase, RNF115
Contact List
Matthias Trost
contact affiliationBiosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, United Kingdom
contact emailmatthias.trost@ncl.ac.uk
lab head
Matthias Trost
contact affiliationNewcastle University
contact emailmatthias.trost@ncl.ac.uk
dataset submitter
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Dataset FTP location
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