PXD026750 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A reference database of electrophoretic migration patterns for human proteins in a set of cell lines. |
Description | Protein denaturing electrophoresis based on sodium dodecyl sulfate (SDS-PAGE) is a technique widely applied for studying proteins, either alone or in combination with specific antibody-based detection. Despite its popularity, SDS-PAGE and western blotting are poorly reproducible and can give different results depending on experimental factors affecting electrophoresis but also on the characteristics of the antibodies used for detection. We reasoned that a reference database of accurately determined migration patterns for human proteins would be very useful for the community. Using mass spectrometry and an internal calibration strategy, we have constructed a database of migration patterns for thousands of proteins in five commonly used human cell lines and one mouse cell line. We show that the electrophoretic molecular weights determined are accurate and, for a large portion of the proteome, highly reproducible across replicates and cell lines. Data exploration highlighted numerous known protein processing events that can be mapped by combining migration patterns and the sequence coverage observed. The information is freely available in a website and should constitute a useful reference for investigators trying to validate western blot results and protein migrations in general. |
HostingRepository | PRIDE |
AnnounceDate | 2023-01-06 |
AnnouncementXML | Submission_2023-01-05_21:13:39.154.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | ManfredoQuadroni |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-06-16 09:36:13 | ID requested | |
⏵ 1 | 2023-01-05 21:13:39 | announced | |
2 | 2023-11-14 07:25:33 | announced | 2023-11-14: Updated project metadata. |
Publication List
Mylonas R, Potts A, Waridel P, Barblan J, Conde Rubio MDC, Widmann C, Quadroni M, A Database of Accurate Electrophoretic Migration Patterns for Human Proteins. J Mol Biol, 435(4):167933(2023) [pubmed] |
Keyword List
submitter keyword: geLC-MS, SDS-PAGE, molecular weight,electrophoresis, western blot |
Contact List
ManfredoQuadroni |
contact affiliation | Protein Analysis Facility, Faculty of Biology and Medicine, University of Lausanne, Switzerland |
contact email | manfredo.quadroni@unil.ch |
lab head | |
ManfredoQuadroni |
contact affiliation | University of Lausanne |
contact email | manfredo.quadroni@unil.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/01/PXD026750 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD026750
- Label: PRIDE project
- Name: A reference database of electrophoretic migration patterns for human proteins in a set of cell lines.