PXD026712-1

PXD026712 is an original dataset announced via ProteomeXchange.

Title	A novel method of spontaneously-released extracellular vesicle isolation from brain
Description	Extracellular Vesicles (EVs) exhibit great potential in for brain disease diagnositics and treatment, representing a valuable tool in the new era of pPrecision medicine which demands high-quality human biospecimens. However, current brain exosome EV isolation approaches rely on its tissue dissociation, which may can contaminate the exosome EV fractions with immature intralumenalcellular vesicles that wcould otherwise be targeted for endolysosomal degradation. Hereby, we present an efficient exosome purification method that captures a more physiologically relevant EV population exosome-enriched extracellular vesicles (EVs) spontaneously released by mouse and human brain tissue, representing a more physiologically relevant exosome EV population.
HostingRepository	PRIDE
AnnounceDate	2023-08-03
AnnouncementXML	Submission_2023-08-03_03:59:48.309.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	MartinaSamiotaki
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-15 06:07:41	ID requested
⏵ 1	2023-08-03 03:59:48	announced
2	2023-11-14 06:58:27	announced	2023-11-14: Updated project metadata.

submitter keyword: exosomes,extracellular vesicles, brain, human, spontaneous release, mouse

IoannisSotiropoulos
contact affiliation	Life and Health Sciences Research Institute (ICVS), Medical School, University of Minho, Braga, Portugal
contact email	ioannis@alunos.uminho.pt
lab head
MartinaSamiotaki
contact affiliation	Protein Analysis Laboratory B.S.R.C. "Alexander Fleming", Alexander Fleming Street 34 16672, Vari, Greece
contact email	samiotaki@fleming.gr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/08/PXD026712

PRIDE project URI

• PRIDE
  1. PXD026712
     1. Label: PRIDE project
     2. Name: A novel method of spontaneously-released extracellular vesicle isolation from brain