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PXD026704-1

PXD026704 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCoating and corruption of human neutrophils by bacterial outer membrane vesicles
DescriptionPorphyromonas gingivalis is a keystone oral pathogen that successfully manipulates the human innate immune defenses, resulting in a chronic pro-inflammatory state of periodontal tissues and beyond. Here we demonstrate that secreted outer membrane vesicles (OMVs) are deployed by P. gingivalis to selectively coat and activate human neutrophils, thereby provoking degranulation without neutrophil killing. Secreted granule components with antibacterial activity, especially LL-37 and MPO, are subsequently degraded by potent OMV-bound proteases known as gingipains, thereby ensuring bacterial survival. In contrast to neutrophils, the P. gingivalis OMVs are efficiently internalized by macrophages and epithelial cells. Importantly, we show that neutrophil coating is a conserved feature displayed by OMVs of at least one other oral pathogen, namely Aggregatibacter actinomycetemcomitans. Altogether, we conclude that P. gingivalis deploys its OMVs for a neutrophil-deceptive strategy to escape phagocytosis and to create a favorable inflammatory niche.
HostingRepositoryPRIDE
AnnounceDate2022-08-16
AnnouncementXMLSubmission_2022-08-16_13:14:47.466.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterClaudia Hirschfeld
SpeciesList scientific name: Porphyromonas gingivalis W83; NCBI TaxID: 242619; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue; deamidated residue; L-citrulline
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-06-14 23:50:41ID requested
12022-08-16 13:14:47announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: OMVs, Porphyromonas gingivalis, neutrophils, myeloperoxidase, citrullination, gingipains, LL-37
Contact List
Prof. Dr. Dörte Becher
contact affiliationUniversity of Greifswald, Center for Functional Genomics of Microbes, Institute for Microbiology, Department of Microbial Proteomics, Greifswald, Germany
contact emaildbecher@uni-greifswald.de
lab head
Claudia Hirschfeld
contact affiliationUniversity of Greifswald
contact emailclaudia.hirschfeld@uni-greifswald.de
dataset submitter
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Dataset FTP location
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