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PXD026699 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSynaptotagmin 13 drives pancreatic endocrine cell egression and islet morphogenesis: BioID
DescriptionIdentification of protein intreactions for the synaptotagmin 13 protein from MDCK cells by BioID-based proximity labelling and LFQ mass spectrometry. Epithelial cell egression is important for organ development and cell differentiation, but also drives cancer metastasis. The tightly connected pancreatic epithelial differentiation and morphogenesis generate islets of Langerhans. However, the morphogenetic drivers and molecular mechanisms are largely unresolved. Here we identify the uncharacterized Synaptotagmin 13 (Syt13) as a major regulator of endocrine cell egression and islet morphogenesis and differentiation. We detected upregulation of Syt13 in endocrine precursors that associates with increased expression of several unique cytoskeletal components. High-resolution imaging reveals a previously unidentified apical-basal to front-rear repolarization during endocrine cell egression. Strikingly, Syt13 directly interacts with acetylated tubulin and phosphoinositide phospholipids to be recruited to the leading edge of egressing cells. Knockout of Syt13 discloses the impairment in endocrine cell egression and skews the α-to-β-cell ratio. At mechanistic levels, Syt13 regulates protein endocytosis to remodel the basement membrane and modulate cell-matrix adhesion at the leading edge of egressing endocrine cells. Altogether, these findings implicate that Ca2+-independent atypical Syt13 vesicular protein functions in regulating cell polarity to orchestrate endocrine cell egression and tissue morphogenesis.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKarsten Boldt
SpeciesList scientific name: Canis lupus x Canis lupus familiaris; NCBI TaxID: 990119;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-06-14 10:29:56ID requested
12022-06-23 07:32:09announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Synaptotagmin 13, proximity labelling, BioID, protein interactions
Contact List
Dr. Karsten Boldt
contact affiliationMedical Bioanalytics Institute for Ophthalmic Research Eberhard-Karls University of Tuebingen
contact emailkarsten.boldt@uni-tuebingen.de
lab head
Karsten Boldt
contact affiliationMedical Bioanalytics
contact emailkarsten.boldt@uni-tuebingen.de
dataset submitter
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Dataset FTP location
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