<<< Full experiment listing

PXD026653-1

PXD026653 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAnaerobic peroxisomes in Entamoeba histolytica metabolize myo-inositol
DescriptionE. histolytica is believed to be devoid of peroxisomes, like most anaerobic protists. In this work, we provided the first evidence that peroxisomes are present in E. histolytica, although the set of proteins (peroxins) responsible for peroxisome biogenesis was reduced to only seven members (Pex1, Pex6, Pex5, Pex11, Pex14, Pex16, and Pex19). Targeting matrix proteins to peroxisomes is reduced to the PTS1-dependent pathway mediated via the soluble Pex5 receptor, while the PTS2 receptor Pex7 is absent. Immunofluorescence microscopy showed that peroxisomal markers (Pex5, Pex14, Pex16, Pex19) are present in vesicles distinct from mitosomes, the endoplasmic reticulum and the endosome/phagosome system, except Pex11, which has dual localization in peroxisomes and mitosomes. Immunoelectron microscopy revealed that Pex14 localized to vesicles of approximately 90-100 nm in diameter. Proteomic analyses of affinity-purified peroxisomes and in silico PTS1 predictions provided datasets of 655 and 56 peroxisomal candidates, respectively; however, only six proteins were shared by both datasets, including myo-inositol dehydrogenase (myo-IDH). Peroxisomal NAD-dependent myo-IDH appeared to be a dimeric enzyme with high affinity to myo-inositol (Km 0,044 mM) and can utilize also scyllo-inositol, D-glucose and D-xylose as substrates. Phylogenetic analyses revealed that orthologs of myo-IDH with PTS1 are present in E. dispar, E. nutalli and E. moshkovskii but not in E. invadens, and form a monophyletic clade of mostly peroxisomal orthologs with free-living M. balamuthi and P. schiedti. The presence of peroxisomes in E. histolytica and other archamoebae breaks the paradigm of peroxisome absence in anaerobes and provides a new potential target for the development of antiparasitic drugs.
HostingRepositoryPRIDE
AnnounceDate2021-10-29
AnnouncementXMLSubmission_2021-10-29_07:36:53.106.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKarel Harant
SpeciesList scientific name: Entamoeba histolytica; NCBI TaxID: 5759;
ModificationListmethylthiolated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-06-11 07:24:31ID requested
12021-10-29 07:36:53announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Entamoeba histolytica, peroxisomes, PTS1, inositol dehydrogenase, PEX
Contact List
prof. RNDr. Jan Tachezy, Ph.D.
contact affiliationDepartment of Parasitology, Faculty of Science, Charles University, BIOCEV, Prague, Czech Republic ORCID logohttp://orcid.org/0000-0001-6976-8446
contact emailjan.tachezy@natur.cuni.cz
lab head
Karel Harant
contact affiliationCharles University
contact emailharant@natur.cuni.cz
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/10/PXD026653
PRIDE project URI
Repository Record List
[ + ]