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PXD026617-1

PXD026617 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTargeted validation of interaction between ErbB4 JM-a isoform and β1 integrin
DescriptionThe ErbB4 receptor isoforms JM-a and JM-b differ within their extracellular juxtamembrane (eJM) domains. Here we used ErbB4 isoforms as a model to address whether structural variation in the eJM domain of receptor tyrosine kinases (RTK) affects downstream signaling. Analysis of ErbB4 interactome and super-resolution cell imaging indicated association of the JM-a- and JM-b-like receptors with specific signaling complexes at different cell surface compartments. A JM-a-specific sequence motif was discovered, and its presence in the eJM domains of several other human RTKs predicted selective STAT activation indicating a conserved RTK signaling mechanism. The RTKs with the JM-a-like eJM motif activated STAT5a, and those that were JM-b-like (lacking the JM-a-like motif) activated STAT5b and STAT3. TYK2 was found to be necessary for JM-b-stimulated STAT5b activation. The activation of STAT5a by the JM-a-like receptors, in turn, involved specific interaction with oligosaccharides N-linked to cell surface glycoproteins such as β1 integrin. These findings provide evidence for a mechanism linking a ubiquitous extracellular motif in RTKs with selective intracellular STAT signaling.
HostingRepositoryPanoramaPublic
AnnounceDate2022-10-22
AnnouncementXMLSubmission_2022-10-22_22:33:13.759.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterJohannes Merilahti
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-06-10 06:53:26ID requested
12022-10-22 22:33:14announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: ErbB4, JM-a, JM-b, STAT signaling, Receptor tyrosine kinase
Contact List
Klaus Elenius
contact affiliationMedicity Research Laboratories and Institute of Biomedicine, Faculty of Medicine, University of Turku, Finland. Turku Bioscience Centre, University of Turku and Åbo Akademi University, Finland
contact emailklaus.elenius@utu.fi
lab head
Johannes Merilahti
contact affiliationTurku Bioscience Centre, University of Turku and Åbo Akademi University, Finland
contact emailjohannes.merilahti@utu.fi
dataset submitter
Full Dataset Link List
Panorama Public dataset URI