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PXD026605-1

PXD026605 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleListeria monocytogenes utilizes the ClpP1/2 proteolytic machinery for fine-tuned substrate degradation under heat stress
DescriptionListeria monocytogenes exhibits two ClpP isoforms (ClpP1/ClpP2) which assemble into a heterooligomeric complex with enhanced proteolytic activity. Herein, we demonstrate that the formation of this complex depends on temperature and reaches a maximum ratio of about 1:1 at heat shock conditions, while almost no complex formation occurred below 4°C. In order to decipher the role of the two isoforms at elevated temperatures, we constructed L. monocytogenes ClpP1, ClpP2 and ClpP1/2 knockout strains and analyzed their protein regulation in comparison to the wild type (WT) strain via whole proteome mass-spectrometry (MS) at 37 °C and 42 °C. While ΔclpP1 strain only altered the expression of very few proteins, ΔclpP2 and ΔclpP1/2 strains revealed the dysregulation of many proteins at both temperatures. These effects were corroborated by crosslinking co-immunoprecipitation MS analysis. Thus, while ClpP1 serves as a mere enhancer of protein degradation in the heterocomplex, ClpP2 is essential for ClpX binding and thus functions as a gatekeeper for substrate entry. Applying an integrated proteomic approach combining whole proteome and co immunoprecipitation datasets, several putative ClpP2 substrates were identified in the context of different temperatures and discussed with regards to their function in cellular pathways such as the SOS response.
HostingRepositoryPRIDE
AnnounceDate2022-09-20
AnnouncementXMLSubmission_2022-09-20_10:20:51.293.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKonstantin Eckel
SpeciesList scientific name: Listeria monocytogenes EGD-e; NCBI TaxID: 169963;
ModificationListiodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-06-09 22:56:23ID requested
12022-09-20 10:20:51announced
22023-11-14 08:52:26announced2023-11-14: Updated project metadata.
Publication List
10.1039/D2CB00077F;
Keyword List
submitter keyword: Listeria monocytogenes, proteolysis, XL-co-IP, ClpP, whole proteome
Contact List
Stephan Axel Sieber
contact affiliationDepartment of Chemistry, Chair of Organic Chemistry II, Center for Functional Protein Assemblies (CPA), Technical University of Munich, Ernst-Otto-Fischer Straße 8, 85748 Garching, Germany
contact emailstephan.sieber@tum.de
lab head
Konstantin Eckel
contact affiliationChair of Organic Chemistry II Technical University of Munich
contact emailk.eckel@tum.de
dataset submitter
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