PXD026519

PXD026519 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	m6A binding proteins identified by SPIDER assay
Description	To assess whether SPIDER could identify modified nucleic acids binding proteins within a complex environment, such as the cellular milieu, we sought to identify N6-methyladenosine (m6A) binding proteins within a total cell lysate. We performed SPIDER assay by incubating biotin-ssRNAs（with and without m6A modification）with the total lysate of YTDHFs (YTHDF1,YTHDF2 and YTHDF3)-overexpressed HEK293T cells
HostingRepository	iProX
AnnounceDate	2022-11-02
AnnouncementXML	Submission_2023-05-06_00:11:48.756.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xuening Wang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-06 22:22:41	ID requested
1	2021-06-06 22:23:06	announced
⏵ 2	2023-05-06 00:11:49	announced	2023-05-06: Update publication information.

Publication List

Jiang HW, Chen H, Zheng YX, Wang XN, Meng Q, Xie J, Zhang J, Zhang C, Xu ZW, Chen ZQ, Wang L, Kong WS, Zhou K, Ma ML, Zhang HN, Guo SJ, Xue JB, Hou JL, Liu ZY, Niu WX, Wang FJ, Wang T, Li W, Wang RN, Dang YJ, Czajkowsky DM, Pei J, Dong JJ, Tao SC, Specific pupylation as IDEntity reporter (SPIDER) for the identification of protein-biomolecule interactions. Sci China Life Sci, 66(8):1869-1887(2023) [pubmed]

Jiang HW, Chen H, Zheng YX, Wang XN, Meng Q, Xie J, Zhang J, Zhang C, Xu ZW, Chen ZQ, Wang L, Kong WS, Zhou K, Ma ML, Zhang HN, Guo SJ, Xue JB, Hou JL, Liu ZY, Niu WX, Wang FJ, Wang T, Li W, Wang RN, Dang YJ, Czajkowsky DM, Pei J, Dong JJ, Tao SC, Specific pupylation as IDEntity reporter (SPIDER) for the identification of protein-biomolecule interactions. Sci China Life Sci, 66(8):1869-1887(2023) [pubmed]

Keyword List

submitter keyword: N6-methyladenosine (m6A), human proteome

submitter keyword: N6-methyladenosine (m6A), human proteome

Contact List

Shengce Tao
contact affiliation	Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University
contact email	taosc@sjtu.edu.cn
lab head
Xuening Wang
contact affiliation	Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University
contact email	wxnyoyo@sjtu.edu.cn
dataset submitter

Full Dataset Link List

iProX dataset URI

iProX dataset URI