Update publication information. To assess whether SPIDER could identify modified nucleic acids binding proteins within a complex environment, such as the cellular milieu, we sought to identify N6-methyladenosine (m6A) binding proteins within a total cell lysate. We performed SPIDER assay by incubating biotin-ssRNAs（with and without m6A modification）with the total lysate of YTDHFs (YTHDF1,YTHDF2 and YTHDF3)-overexpressed HEK293T cells