PXD026409

PXD026409 is an original dataset announced via ProteomeXchange.

Title	MEST Promotes Lung Cancer Invasion and Metastasis by Interacting with VCP to Activate NF-κB Signaling
Description	Active cell invasion is a hallmark of metastatic cancer, leading to unfavorable clinical outcome. We here established high-invasive lung cancer cell models, A549-i8 and H1299-i8, and identified mesoderm-specific transcript (MEST) as a novel invasive regulator for lung cancer. MEST overexpression promoted the metastasis of lung cancer cells in vivo and in vitro by activating NF-κB signaling. MEST enhanced the interaction of VCP with IκBα, which accelerated IκBα degradation and NF-κB activation. Such an acceleration could be abrogated by VCP silencing, indicating that MEST is an upstream regulator to induce the sequential VCP/IκBα/NF-κB signaling. Furthermore, high MEST and VCP expressions were associated with poor survival of lung cancer patients. Collectively, we demonstrated that MEST plays an important role in driving invasion and metastasis of lung cancer by interacting with VCP, coordinating IκBα/NF-κB pathway. Targeting MEST/VCP/IκBα/NF-κB signaling may represent a promising therapeutic strategy for lung cancer.
HostingRepository	iProX
AnnounceDate	2021-06-02
AnnouncementXML	Submission_2022-11-14_23:55:45.507.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jing Zhang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap; Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-01 22:06:26	ID requested
⏵ 1	2022-11-14 23:55:46	announced

Wang Y, Zhang J, Li YJ, Yu NN, Liu WT, Liang JZ, Xu WW, Sun ZH, Li B, He QY, B signaling. J Exp Clin Cancer Res, 40(1):301(2021) [pubmed]

submitter keyword: Lung cancer, MEST, VCP, NF-κB, Metastasis

Yang Wang
contact affiliation	Jinan University
contact email	wangyang8857@gmail.com
lab head
Jing Zhang
contact affiliation	Jinan University
contact email	zj6410@jnu.edu.cn
dataset submitter

iProX dataset URI