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PXD026354-1

PXD026354 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHeart and skeletal muscle of young adult and early aging mice
DescriptionLeft cardiac ventricles and quadriceps femoris muscles were collected from young adult (16-17 wk) and early aging (86 wk) C57BL6/J mice (n=4, 2 male 2 female). Proteins were extracted in 200 µL RIPA buffer and Halt protease/phosphatase inhibitor with a hand-held sonicator followed by centrifugation, reduced with dithiothreitol, alkylated with iodoacetamide, then digested with modified sequencing grade trypsin overnight at 37degC using a filter-assisted sample preparation protocol. The peptides were fractionated using Thermo high-pH reversed phase columns into 8 fractions. Label-free bottom-up mass spectrometry was performed using data dependent acquisition on a Thermo Orbitrap Q-Exactive HF instrument using typical settings.
HostingRepositoryjPOST
AnnounceDate2021-07-23
AnnouncementXMLSubmission_2021-07-23_09:50:36.522.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterEdward Lau
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListS-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; alpha-amino acetylated residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-05-28 14:25:36ID requested
12021-07-23 09:50:36announced
22022-09-18 03:28:51announced2022-09-18: Updated FTP location.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: mouse, label free, heart, skeletal muscle, aging
Contact List
Edward Lau
lab head
Edward Lau
contact affiliationUniversity of Colorado
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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