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PXD026343-1

PXD026343 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTensin3 interaction with talin drives formation of fibronectin-associated fibrillar adhesions
DescriptionThe formation of healthy tissue involves continuous remodelling of the extracellular matrix (ECM). Whilst it is known that this requires integrin-associated cell-ECM adhesion sites (CMAs) and actomyosin-mediated forces, the underlying mechanisms remain unclear. Here we examine how tensin3 contributes to formation of fibrillar adhesions (FBs) and fibronectin fibrillogenesis. Using BioID mass spectrometry and a mitochondrial targeting assay, we establish that tensin3 associates with the mechanosensors talin and vinculin. We show that the talin R11 rod domain binds directly to a helical motif within the central intrinsically disordered region (IDR) of tensin3, whilst vinculin binds indirectly to tensin3 via talin. Using CRISPR knock-out cells in combination with defined tensin3 mutations, we show (i) that tensin3 is critical for formation of alpha5 beta1-integrin FBs and for fibronectin fibrillogenesis, and (ii) the talin/tensin3 interaction drives this process, with vinculin acting to potentiate it.
HostingRepositoryPRIDE
AnnounceDate2022-09-09
AnnouncementXMLSubmission_2022-09-09_03:11:16.185.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSuat Peng Neo
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-05-28 02:48:38ID requested
12022-09-09 03:11:16announced
22023-11-14 08:53:01announced2023-11-14: Updated project metadata.
Publication List
10.1083/JCB.202107022;
Keyword List
submitter keyword: tensin, talin, vinculin, fibronectin, extracellular matrix
Contact List
Jayantha Gunaratne
contact affiliationInstitute of Molecular and Cell Biology, Agency for Science, Technology and Research, TBP Lab
contact emailjayanthag@imcb.a-star.edu.sg
lab head
Suat Peng Neo
contact affiliationInstitute of Molecular and Cell Biology (ASTAR)
contact emailneosp@imcb.a-star.edu.sg
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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