PXD026284-2
PXD026284 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mass spectrometry analysis of FLAG-5MP complexes |
Description | FLAG-tagged 5MP (h5MP1/BZW2 from human and 5MP/Kra from fly) was ectopically expressed in relevant host cells (HEK293T for human and S2 for fly) by transient transfection, and one-step affinity-purified by anti-FLAG affinity resin.The one-step affinity purification products were resolved by SDS-PAGE, silver-stained and subjected for MS after dividing the whole lane into 12-15 fractions. |
HostingRepository | jPOST |
AnnounceDate | 2021-05-27 |
AnnouncementXML | Submission_2022-03-23_21:29:43.981.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Naoki Tani |
SpeciesList | scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227; |
ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; deamidated L-asparagine; deamidated L-glutamine |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2021-05-26 05:25:41 | ID requested | |
1 | 2021-05-26 19:37:58 | announced | |
⏵ 2 | 2022-03-23 21:29:44 | announced | 2022-03-24: Updated PubMed. |
3 | 2022-09-18 03:02:48 | announced | 2022-09-18: Updated FTP location. |
Publication List
Singh CR, Glineburg MR, Moore C, Tani N, Jaiswal R, Zou Y, Aube E, Gillaspie S, Thornton M, Cecil A, Hilgers M, Takasu A, Asano I, Asano M, Escalante CR, Nakamura A, Todd PK, Asano K, Human oncoprotein 5MP suppresses general and repeat-associated non-AUG translation via eIF3 by a common mechanism. Cell Rep, 36(2):109376(2021) [pubmed] |
Keyword List
submitter keyword: eIF5-mimic protein (5MP) |
Contact List
Katsura Asano | |
---|---|
lab head | |
Naoki Tani | |
contact affiliation | Kumamoto university |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.biosciencedbc.jp/archive/jpostrepos/JPST001188 |