PXD026284

PXD026284 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Mass spectrometry analysis of FLAG-5MP complexes
Description	FLAG-tagged 5MP (h5MP1/BZW2 from human and 5MP/Kra from fly) was ectopically expressed in relevant host cells (HEK293T for human and S2 for fly) by transient transfection, and one-step affinity-purified by anti-FLAG affinity resin.The one-step affinity purification products were resolved by SDS-PAGE, silver-stained and subjected for MS after dividing the whole lane into 12-15 fractions.
HostingRepository	jPOST
AnnounceDate	2021-05-27
AnnouncementXML	Submission_2022-09-18_03:02:47.446.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Naoki Tani
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; deamidated L-asparagine; deamidated L-glutamine
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-26 05:25:41	ID requested
1	2021-05-26 19:37:58	announced
2	2022-03-23 21:29:44	announced	2022-03-24: Updated PubMed.
⏵ 3	2022-09-18 03:02:48	announced	2022-09-18: Updated FTP location.

Publication List

Singh CR, Glineburg MR, Moore C, Tani N, Jaiswal R, Zou Y, Aube E, Gillaspie S, Thornton M, Cecil A, Hilgers M, Takasu A, Asano I, Asano M, Escalante CR, Nakamura A, Todd PK, Asano K, Human oncoprotein 5MP suppresses general and repeat-associated non-AUG translation via eIF3 by a common mechanism. Cell Rep, 36(2):109376(2021) [pubmed]

Singh CR, Glineburg MR, Moore C, Tani N, Jaiswal R, Zou Y, Aube E, Gillaspie S, Thornton M, Cecil A, Hilgers M, Takasu A, Asano I, Asano M, Escalante CR, Nakamura A, Todd PK, Asano K, Human oncoprotein 5MP suppresses general and repeat-associated non-AUG translation via eIF3 by a common mechanism. Cell Rep, 36(2):109376(2021) [pubmed]

Keyword List

submitter keyword: eIF5-mimic protein (5MP)

submitter keyword: eIF5-mimic protein (5MP)

Contact List

Katsura Asano
lab head
Naoki Tani
contact affiliation	Kumamoto university
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001188/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001188/