PXD026220 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | SILAKin: A novel high throughput SILAC-based quantitative mass spectrometry assay to identify the substratome of kinases secreted by pathogens |
Description | We developed SILAkin, a novel and easy method to identify substrates that is applicable to most kinases. It combines phosphatase treatment, pulse heating, in vitro kinase assay and SILAC (Stable Isotope Labeling with Amino acids in Cell culture)-based quantitative mass spectrometry (MS). SILAkin was developed using the Leishmania casein kinase 1 (L-CK1.2) as experimental model. Leishmania, an intracellular parasite causing Leishmaniasis, releases L-CK1.2 in its host cell. Applying this novel assay allowed us to gain unprecedented insight into host-pathogen interactions through the identification of 225 host substrates phosphorylated by L-CK1.2. The substratome was validated experimentally by L-CK1.2 and human CK1δ, demonstrating the efficiency of SILAkin to identify new substrates and revealing novel regulatory pathways. Finally, SILAkin was instrumental in highlighting host pathways potentially regulated by L-CK1.2 in Leishmania-infected host cells. |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-17 |
AnnouncementXML | Submission_2022-02-17_04:15:58.644.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Valentin SABATET |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-05-24 03:47:31 | ID requested | |
⏵ 1 | 2022-02-17 04:15:59 | announced | |
Publication List
Smirlis D, Dingli F, Sabatet V, Roth A, Knippschild U, Loew D, Sp, ä, th GF, Rachidi N, -Secreted Casein Kinase 1 Using a SILAC-Based Quantitative Mass Spectrometry Assay. Front Cell Dev Biol, 9():800098(2021) [pubmed] |
Keyword List
submitter keyword: Casein kinase 1, substrate screen, Leishmania, host-pathogen interactions |
Contact List
Damarys Loew |
contact affiliation | Head of the mass spectrometry platform of the Institut Curie |
contact email | damarys.loew@curie.fr |
lab head | |
Valentin SABATET |
contact affiliation | Curie Institute |
contact email | valentin.sabatet@curie.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD026220
- Label: PRIDE project
- Name: SILAKin: A novel high throughput SILAC-based quantitative mass spectrometry assay to identify the substratome of kinases secreted by pathogens