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PXD026110-1

PXD026110 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleFASN-dependent de novo lipogenesis is required for brain development
DescriptionFate and behaviour of neural progenitor cells is tightly regulated during mammalian brain development. Metabolic pathways, such as glycolysis and oxidative phosphorylation, that are required for supplying energy and providing molecular building blocks to generate cells, govern progenitor function. However, the role of de novo lipogenesis, which is the conversion of glucose into fatty acids through the multi-enzyme protein fatty acid synthase (FASN), for brain development remains unknown. Using Emx1Cre-mediated, tissue-specific deletion of Fasn in the mouse embryonic telencephalon, we show that loss of FASN causes severe microcephaly, largely due to altered polarity of apical, radial glia progenitors (APs) and reduced progenitor proliferation. Further, genetic deletion and pharmacological inhibition of FASN in human embryonic stem cell (ESC)-derived forebrain organoids identifies a conserved role of FASN-dependent lipogenesis for radial glia cell polarity and progenitor expansion in the developing human forebrain. Thus, our data establish a role of de novo lipogenesis for mouse and human brain development and identify a link between progenitor cell polarity and lipid metabolism.
HostingRepositoryPRIDE
AnnounceDate2022-01-14
AnnouncementXMLSubmission_2022-01-14_13:24:50.698.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDaniel Gonzalez
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; monohydroxylated residue
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-05-20 01:56:40ID requested
12022-01-14 13:24:51announced
Publication List
10.1073/PNAS.2112040119;
Keyword List
submitter keyword: Lipogenesis, FASN, brain development, brain organoids, mouse, human
Contact List
Sebastian Jessberger
contact affiliationUniversity of Zurich, Brain Research Institute, Laboratory of Neural Plasticity
contact emailjessberger@hifo.uzh.ch
lab head
Daniel Gonzalez
contact affiliationUniversity of Zurich
contact emailgonzalez@hifo.uzh.ch
dataset submitter
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Dataset FTP location
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