PXD026098 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Phosphorylation of the NBDY microprotein promotes dissociation of biomolecular condensates |
Description | Proteogenomic identification of translated small open reading frames in human has revealed thousands of microproteins, or polypeptides of fewer than 100 amino acids, that were previously invisible to geneticists. Hundreds of microproteins have been shown to be essential for cell growth and proliferation, and many regulate macromolecular complexes, but the vast majority remain functionally uncharacterized, lack secondary structure, and exhibit limited evolutionary conservation. One such intrinsically disordered microprotein is NBDY, a 68-amino acid component of membraneless organelles known as P-bodies. In this work, we show that NBDY can undergo liquid-liquid phase separation, a biophysical process thought to underlie the formation of membraneless organelles, in the presence of RNA in vitro. Phosphorylation of NBDY drives liquid phase remixing in vitro and macroscopic P-body dissociation in cells undergoing growth factor signaling and cell division. These results suggest that NBDY phosphorylation is a key regulator of P-body dynamics in cells, and more broadly that intrinsically disordered microproteins may contribute to liquid-liquid phase separation and remixing behavior in cells. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:30:13.983.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | ZHENKUN NA |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-05-19 22:27:26 | ID requested | |
1 | 2022-10-14 00:58:56 | announced | |
⏵ 2 | 2023-11-14 08:30:14 | announced | 2023-11-14: Updated project metadata. |
Publication List
Na Z, Luo Y, Cui DS, Khitun A, Smelyansky S, Loria JP, Slavoff SA, Phosphorylation of a Human Microprotein Promotes Dissociation of Biomolecular Condensates. J Am Chem Soc, 143(32):12675-12687(2021) [pubmed] |
Keyword List
submitter keyword: phosphorylation, cell cycle, EGF,NBDY |
Contact List
Sarah Slavoff |
contact affiliation | Department of Chemistry, Yale University |
contact email | sarah.slavoff@yale.edu |
lab head | |
ZHENKUN NA |
contact affiliation | Yale University |
contact email | zhenkun.na@yale.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD026098
- Label: PRIDE project
- Name: Phosphorylation of the NBDY microprotein promotes dissociation of biomolecular condensates