PXD026098

PXD026098 is an original dataset announced via ProteomeXchange.

Title	Phosphorylation of the NBDY microprotein promotes dissociation of biomolecular condensates
Description	Proteogenomic identification of translated small open reading frames in human has revealed thousands of microproteins, or polypeptides of fewer than 100 amino acids, that were previously invisible to geneticists. Hundreds of microproteins have been shown to be essential for cell growth and proliferation, and many regulate macromolecular complexes, but the vast majority remain functionally uncharacterized, lack secondary structure, and exhibit limited evolutionary conservation. One such intrinsically disordered microprotein is NBDY, a 68-amino acid component of membraneless organelles known as P-bodies. In this work, we show that NBDY can undergo liquid-liquid phase separation, a biophysical process thought to underlie the formation of membraneless organelles, in the presence of RNA in vitro. Phosphorylation of NBDY drives liquid phase remixing in vitro and macroscopic P-body dissociation in cells undergoing growth factor signaling and cell division. These results suggest that NBDY phosphorylation is a key regulator of P-body dynamics in cells, and more broadly that intrinsically disordered microproteins may contribute to liquid-liquid phase separation and remixing behavior in cells.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:30:13.983.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	ZHENKUN NA
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-19 22:27:26	ID requested
1	2022-10-14 00:58:56	announced
⏵ 2	2023-11-14 08:30:14	announced	2023-11-14: Updated project metadata.

Na Z, Luo Y, Cui DS, Khitun A, Smelyansky S, Loria JP, Slavoff SA, Phosphorylation of a Human Microprotein Promotes Dissociation of Biomolecular Condensates. J Am Chem Soc, 143(32):12675-12687(2021) [pubmed]

submitter keyword: phosphorylation, cell cycle, EGF,NBDY

Sarah Slavoff
contact affiliation	Department of Chemistry, Yale University
contact email	sarah.slavoff@yale.edu
lab head
ZHENKUN NA
contact affiliation	Yale University
contact email	zhenkun.na@yale.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD026098

PRIDE project URI

• PRIDE
  1. PXD026098
     1. Label: PRIDE project
     2. Name: Phosphorylation of the NBDY microprotein promotes dissociation of biomolecular condensates